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ACE2 Rabbit pAb (A12737)

Datasheet

ABclonal:Western blot - ACE2 Rabbit pAb (A12737)
ABclonal:Western blot - ACE2 Rabbit pAb (A12737)
ABclonal:Immunohistochemistry - ACE2 Rabbit pAb (A12737)
ABclonal:Immunohistochemistry - ACE2 Rabbit pAb (A12737)
ABclonal:Immunohistochemistry - ACE2 Rabbit pAb (A12737)
ABclonal:Immunofluorescence - ACE2 Rabbit pAb (A12737)
ABclonal:Immunofluorescence - ACE2 Rabbit pAb (A12737)
ABclonal:Immunofluorescence - ACE2 Rabbit pAb (A12737)
ABclonal:Immunofluorescence - ACE2 Rabbit pAb (A12737)

Overview

Product nameACE2 Rabbit pAb
Catalog No.A12737
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 18-250 of human ACE2 (NP_068576.1).
SequenceQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMN
Gene ID59272
Swiss protQ9BYF1
SynonymsACE2; ACEH
Calculated MW63kDa/92kDa
Observed MW135KDa/100KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, Mouse brain, Mouse kidney, Rat brain
Cellular locationCell membrane, Cytoplasm, Secreted, Single-pass type I membrane protein
Customer validation

WB(Homo sapiens)

    ABclonal:Western blot - ACE2 Rabbit pAb (A12737) }

    Western blot - ACE2 Rabbit pAb (A12737)

    Western blot analysis of extracts of various cell lines, using ACE2 antibody (A12737) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - ACE2 Rabbit pAb (A12737) }

    Western blot - ACE2 Rabbit pAb (A12737)

    Western blot analysis of extracts of HepG2 cells, using ACE2 antibody (A12737) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - ACE2 Rabbit pAb (A12737) }

    Immunohistochemistry - ACE2 Rabbit pAb (A12737)

    Immunohistochemistry of paraffin-embedded rat lung using ACE2 Rabbit pAb (A12737) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - ACE2 Rabbit pAb (A12737) }

    Immunohistochemistry - ACE2 Rabbit pAb (A12737)

    Immunohistochemistry of paraffin-embedded human colon carcinoma using ACE2 Rabbit pAb (A12737) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - ACE2 Rabbit pAb (A12737) }

    Immunohistochemistry - ACE2 Rabbit pAb (A12737)

    Immunohistochemistry of paraffin-embedded mouse kidney using ACE2 Rabbit pAb (A12737) at dilution of 1:100 (40x lens).
    ABclonal:Immunofluorescence - ACE2 Rabbit pAb (A12737) }

    Immunofluorescence - ACE2 Rabbit pAb (A12737)

    Immunofluorescence analysis of rat testis using ACE2 Rabbit pAb (A12737) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ACE2 Rabbit pAb (A12737) }

    Immunofluorescence - ACE2 Rabbit pAb (A12737)

    Immunofluorescence analysis of rat kidney using ACE2 Rabbit pAb (A12737) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ACE2 Rabbit pAb (A12737) }

    Immunofluorescence - ACE2 Rabbit pAb (A12737)

    Immunofluorescence analysis of mouse testis using ACE2 Rabbit pAb (A12737) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ACE2 Rabbit pAb (A12737) }

    Immunofluorescence - ACE2 Rabbit pAb (A12737)

    Immunofluorescence analysis of mouse kidney using ACE2 Rabbit pAb (A12737) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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