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ACE2 Rabbit pAb (A9454)

Datasheet

ABclonal:Western blot - ACE2 Polyclonal Antibody (A9454)

Overview

Product nameACE2 Rabbit pAb
Catalog No.A9454
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
ImmunogenA synthetic Peptide of human ACE2
SequenceEmail for sequence
Gene ID59272
Swiss protQ9BYF1
SynonymsACE2; ACEH
Calculated MW63kDa/92kDa
Observed MW95kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50- 1:100
FC 1:20 - 1:50
Storage bufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Flow Cytometry    
Positive samplesHepG2
Cellular locationCell membrane, Cytoplasm, Secreted, Single-pass type I membrane protein
    ABclonal:Western blot - ACE2 Polyclonal Antibody (A9454) }

    Western blot - ACE2 Polyclonal Antibody (A9454)

    Western blot analysis of extracts of HepG2 cells, using ACE2 antibody (A9454).
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.

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