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[KO Validated] HDAC1 Rabbit pAb (A0238)

Reviews (5)Publications (13) Datasheet

ABclonal:Immunohistochemistry - [KO Validated] HDAC1 Rabbit pAb (A0238)

ABclonal:Immunofluorescence - [KO Validated] HDAC1 Rabbit pAb (A0238)

ABclonal:Immunoprecipitation - [KO Validated] HDAC1 Rabbit pAb (A0238)

Review

Overview

Product name	[KO Validated] HDAC1 Rabbit pAb
Catalog No.	A0238
RRID	AB_2757051
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2).
Sequence	SGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKEEVKLA
Gene ID	3065
Swiss prot	Q13547
Synonyms	HD1; RPD3; KDAC1; GON-10; RPD3L1
Calculated MW	55kDa
Observed MW	62kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:100 IP 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	293T, HeLa, HepG2, MCF7, Mouse thymus, Mouse testis, Rat thymus, Rat testis
Cellular location	Nucleus
Customer validation	IF(Human acute leukemia cells) WB(Human acute leukemia cells, HL60,Hut102, 293T, Homo sapiens, Rattus norvegicus, Mus musculus, Sus scrofa) IP(Human acute leukemia cells) IP(Rattus norvegicus) ChIP(Rattus norvegicus)

Research Area

[KO Validated] HDAC1 Rabbit pAb images

Western blot - [KO Validated] HDAC1 Rabbit pAb (A0238)

Western blot analysis of extracts of various cell lines, using HDAC1 antibody (A0238) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot - [KO Validated] HDAC1 Rabbit pAb (A0238)

Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (A0238) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Immunohistochemistry - [KO Validated] HDAC1 Rabbit pAb (A0238)

Immunohistochemistry analysis of paraffin-embedded rat spleen using [KO Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry - [KO Validated] HDAC1 Rabbit pAb (A0238)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using [KO Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry - [KO Validated] HDAC1 Rabbit pAb (A0238)

Immunohistochemistry analysis of paraffin-embedded human colon using [KO Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence - [KO Validated] HDAC1 Rabbit pAb (A0238)

Immunofluorescence analysis of A549 cells using HDAC1 antibody (A0238). Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] HDAC1 Rabbit pAb (A0238)

Confocal immunofluorescence analysis of L929 cells using HDAC1 Polyclonal Antibody (A0238) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunoprecipitation - [KO Validated] HDAC1 Rabbit pAb (A0238)

Immunoprecipitation analysis of 200 μg extracts of HT-29 cells, using 3 μg HDAC1 antibody (A0238). Western blot was performed from the immunoprecipitate using HDAC1 antibody (A0238) at a dilution of 1:1000.

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References for [KO Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KO Validated] HDAC1 Rabbit pAb

Journal:

Neuroscience

Application:

IF:

3.59

Species:

Homo sapiens

PMID:

25779965

Title:

Galangin-induced down-regulation of BACE1 by epigenetic mechanisms in SH-SY5Y cells

References for [KO Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KO Validated] HDAC1 Rabbit pAb

Journal:

Cell Death And Differentiation

Application:

WB, IP, ChIP

IF:

15.828

Species:

Rattus norvegicus

PMID:

28186506

Title:

Epigenetic regulation of HDAC1 SUMOylation as an endogenous neuroprotection against Aβ toxicity in a mouse model of Alzheimer's disease

References for [KO Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KO Validated] HDAC1 Rabbit pAb

Journal:

Oncology reports

Application:

IF:

3.906

Species:

Homo sapiens

PMID:

30542715

Title:

Inhibition of miR‑194 suppresses the Wnt/β‑catenin signalling pathway in gastric cancer.

References for [KO Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KO Validated] HDAC1 Rabbit pAb

Journal:

The Journal of biological chemistry

Application:

IF:

5.157

Species:

Homo sapiens

PMID:

31694914

Title:

SUMOylation down-regulates rDNA transcription by repressing expression of upstream-binding factor and proto-oncogene c-Myc

References for [KO Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KO Validated] HDAC1 Rabbit pAb

Journal:

Cell Death Differ

Application:

IF:

15.828

Species:

Mus musculus

PMID:

33024274

Title:

Histone deacetylase 3 aberration inhibits Klotho transcription and promotes renal fibrosis

References for [KO Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KO Validated] HDAC1 Rabbit pAb

Journal:

Int J Biol Sci

Application:

IF:

6.58

Species:

Mus musculus

PMID:

34803511

Title:

Co-targeting BET bromodomain BRD4 and RAC1 suppresses growth, stemness and tumorigenesis by disrupting the c-MYC-G9a-FTH1axis and downregulating HDAC1 in molecular subtypes of breast cancer

References for [KO Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KO Validated] HDAC1 Rabbit pAb

Journal:

Mol Cancer

Application:

IF:

27.4

Species:

Homo sapiens

PMID:

34863211

Title:

A novel protein AXIN1-295aa encoded by circAXIN1 activates the Wnt/β-catenin signaling pathway to promote gastric cancer progression

References for [KO Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KO Validated] HDAC1 Rabbit pAb

Journal:

Journal of cellular physiology

Application:

IF:

6.38

Species:

Homo sapiens

PMID:

30786008

Title:

Regulation of P300 and HDAC1 on endoplasmic reticulum stress in isoniazid‐induced HL‐7702 hepatocyte injury

References for [KO Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KO Validated] HDAC1 Rabbit pAb

Journal:

J Virol

Application:

IF:

5.103

Species:

Sus scrofa

PMID:

34232065

Title:

Porcine epidemic diarrhea virus inhibits HDAC1 expression to facilitate its replication via binding of its nucleocapsid protein to host transcription factor Sp1

References for [KO Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KO Validated] HDAC1 Rabbit pAb

Journal:

Journal of proteome research

Application:

IF:

4.46

Species:

Homo sapiens

PMID:

28234478

Title:

Large-scale identification of protein crotonylation reveals its role in multiple cellular functions

References for [KO Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KO Validated] HDAC1 Rabbit pAb

Journal:

Molecular medicine reports

Application:

IF:

3.42

Species:

Homo sapiens

PMID:

31974619

Title:

Chidamide induces necroptosis via regulation of c‑FLIPL expression in Jurkat and HUT‑78 cells

References for [KO Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KO Validated] HDAC1 Rabbit pAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Mus musculus

PMID:

35915078

Title:

Nucleosome remodeling and deacetylation complex and MBD3 influence mouse embryonic stem cell naïve pluripotency under inhibition of protein kinase C - PMC

References for [KO Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KO Validated] HDAC1 Rabbit pAb

Journal:

Nature metabolism

Application:

IF:

19.86

Species:

Homo sapiens

PMID:

36593272