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[KO Validated] HDAC1 Rabbit pAb (A0238)

Reviews (5)Publications (11) Datasheet

ABclonal:Western blot - [KO Validated] HDAC1 Rabbit pAb (A0238)
ABclonal:Western blot - [KO Validated] HDAC1 Rabbit pAb (A0238)
ABclonal:Immunohistochemistry - [KO Validated] HDAC1 Rabbit pAb (A0238)
ABclonal:Immunohistochemistry - [KO Validated] HDAC1 Rabbit pAb (A0238)
ABclonal:Immunohistochemistry - [KO Validated] HDAC1 Rabbit pAb (A0238)
ABclonal:Immunofluorescence - [KO Validated] HDAC1 Rabbit pAb (A0238)
ABclonal:Immunofluorescence - [KO Validated] HDAC1 Rabbit pAb (A0238)
ABclonal:Immunoprecipitation - [KO Validated] HDAC1 Rabbit pAb (A0238)
Review
ABclonal: review for [KO Validated] HDAC1 Rabbit pAb(A0238)
Review
ABclonal: review for [KO Validated] HDAC1 Rabbit pAb(A0238)
Review
ABclonal: review for [KO Validated] HDAC1 Rabbit pAb(A0238)
Review
ABclonal: review for [KO Validated] HDAC1 Rabbit pAb(A0238)
Review
ABclonal: review for [KO Validated] HDAC1 Rabbit pAb(A0238)

Overview

Product name[KO Validated] HDAC1 Rabbit pAb
Catalog No.A0238
RRIDAB_2757051
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2).
SequenceSGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKEEVKLA
Gene ID3065
Swiss protQ13547
SynonymsGON-10; HD1; RPD3; RPD3L1; HDAC1
Calculated MW55kDa
Observed MW62kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIF/ICCIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:100 - 1:200
  • IF/ICC 1:50 - 1:100
  • IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples293T, HeLa, HepG2, MCF7, Mouse thymus, Mouse testis, Rat thymus, Rat testis
Cellular locationNucleus
Customer validation

(Human acute leukemia cells, HL60,Hut102, 293T)

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Sus scrofa)

IP(Rattus norvegicus)

ChIP(Rattus norvegicus)

    ABclonal:Western blot - [KO Validated] HDAC1 Rabbit pAb (A0238)}

    Western blot - [KO Validated] HDAC1 Rabbit pAb (A0238)

    Western blot analysis of extracts of various cell lines, using HDAC1 antibody (A0238) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - [KO Validated] HDAC1 Rabbit pAb (A0238)}

    Western blot - [KO Validated] HDAC1 Rabbit pAb (A0238)

    Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (A0238) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - [KO Validated] HDAC1 Rabbit pAb (A0238)}

    Immunohistochemistry - [KO Validated] HDAC1 Rabbit pAb (A0238)

    Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - [KO Validated] HDAC1 Rabbit pAb (A0238)}

    Immunohistochemistry - [KO Validated] HDAC1 Rabbit pAb (A0238)

    Immunohistochemistry of paraffin-embedded human lung cancer using [KO Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - [KO Validated] HDAC1 Rabbit pAb (A0238)}

    Immunohistochemistry - [KO Validated] HDAC1 Rabbit pAb (A0238)

    Immunohistochemistry of paraffin-embedded human colon using [KO Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - [KO Validated] HDAC1 Rabbit pAb (A0238)}

    Immunofluorescence - [KO Validated] HDAC1 Rabbit pAb (A0238)

    Immunofluorescence analysis of A549 cells using HDAC1 antibody (A0238). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - [KO Validated] HDAC1 Rabbit pAb (A0238)}

    Immunofluorescence - [KO Validated] HDAC1 Rabbit pAb (A0238)

    Confocal immunofluorescence analysis of L929 cells using HDAC1 Polyclonal Antibody (A0238) at dilution of 1:200. Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - [KO Validated] HDAC1 Rabbit pAb (A0238)}

    Immunoprecipitation - [KO Validated] HDAC1 Rabbit pAb (A0238)

    Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug HDAC1 antibody (A0238). Western blot was performed from the immunoprecipitate using HDAC1 antibody (A0238) at a dilution of 1:1000.

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