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IFNAR2 Rabbit pAb (A1769)

Publication (1) Datasheet

ABclonal:Western blot - IFNAR2 Rabbit pAb (A1769)
ABclonal:Immunohistochemistry - IFNAR2 Rabbit pAb (A1769)
ABclonal:Immunohistochemistry - IFNAR2 Rabbit pAb (A1769)
ABclonal:Immunohistochemistry - IFNAR2 Rabbit pAb (A1769)
ABclonal:Immunohistochemistry - IFNAR2 Rabbit pAb (A1769)
ABclonal:Immunofluorescence - IFNAR2 Rabbit pAb (A1769)

Overview

Product nameIFNAR2 Rabbit pAb
Catalog No.A1769
RRIDAB_2763812
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Multiple transcript variants encoding at least two different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 101-243 of human IFNAR2 (NP_997468.1).
SequenceSTHEAYVTVLEGFSGNTTLFSCSHNFWLAIDMSFEPPEFEIVGFTNHINVMVKFPSIVEEELQFDLSLVIEEQSEGIVKKHKPEIKGNMSGNFTYIIDKLIPNTNYCVSVYLEHSDEQAVIKSPLKCTLLPPGQESESAESAK
Gene ID3455
Swiss protP48551
SynonymsIFNAR2; IFN-R; IFN-alpha-REC; IFNABR; IFNARB; IMD45
Calculated MW27kDa/37kDa/57kDa
Observed MW58KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIF/ICCIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMCF7, Raji, Mouse liver, Rat liver
Cellular locationMembrane, Secreted, Single-pass type I membrane protein
Customer validation

WB(Homo sapiens)

    ABclonal:Western blot - IFNAR2 Rabbit pAb (A1769)}

    Western blot - IFNAR2 Rabbit pAb (A1769)

    Western blot analysis of extracts of various cell lines, using IFNAR2 antibody (A1769) at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunohistochemistry - IFNAR2 Rabbit pAb (A1769)}

    Immunohistochemistry - IFNAR2 Rabbit pAb (A1769)

    Immunohistochemistry of paraffin-embedded Human uterine cancer using IFNAR2 antibody (A1769) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - IFNAR2 Rabbit pAb (A1769)}

    Immunohistochemistry - IFNAR2 Rabbit pAb (A1769)

    Immunohistochemistry of paraffin-embedded Human placenta using IFNAR2 antibody (A1769) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - IFNAR2 Rabbit pAb (A1769)}

    Immunohistochemistry - IFNAR2 Rabbit pAb (A1769)

    Immunohistochemistry of paraffin-embedded Mouse heart using IFNAR2 antibody (A1769) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - IFNAR2 Rabbit pAb (A1769)}

    Immunohistochemistry - IFNAR2 Rabbit pAb (A1769)

    Immunohistochemistry of paraffin-embedded Rat heart using IFNAR2 antibody (A1769) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - IFNAR2 Rabbit pAb (A1769)}

    Immunofluorescence - IFNAR2 Rabbit pAb (A1769)

    Immunofluorescence analysis of Raji cells using IFNAR2 Rabbit pAb (A1769) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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