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PSMB10 Rabbit pAb (A5452)

Publication (1) Datasheet

ABclonal:Western blot - PSMB10 Polyclonal Antibody (A5452)
ABclonal:Immunohistochemistry - PSMB10 Polyclonal Antibody (A5452)
ABclonal:Immunohistochemistry - PSMB10 Polyclonal Antibody (A5452)
ABclonal:Immunofluorescence - PSMB10 Polyclonal Antibody (A5452)

Overview

Product namePSMB10 Rabbit pAb
Catalog No.A5452
RRIDAB_2766253
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human PSMB10 (NP_002792.1).
SequenceMLKPALEPRGGFSFENCQRNASLERVLPGLKVPHARKTGTTIAGLVFQDGVILGADTRATNDSVVADKSCEKIHFIAPKIYCCGAGVAADAEMTTRMVASKMELHALSTGREPRVATVTRILRQTLFRYQGHVGASLIVGGVDLTGPQLYGVHPHGSYSRLPFTALGSGQDAALAVLEDRFQPNMTLEAAQGLLVEAVTAGILGDLGSGGNVDACVITKTGAKLLRTLSSPTEPVKRSGRYHFVPGTTAVLTQTVKPLTLELVEETVQAMEVE
Gene ID5699
Swiss protP40306
SynonymsPSMB10; LMP10; MECL1; beta2i
Calculated MW28kDa
Observed MW29kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIF/ICCIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesTHP-1
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Macaca mulatta)

    ABclonal:Western blot - PSMB10 Polyclonal Antibody (A5452) }

    Western blot - PSMB10 Polyclonal Antibody (A5452)

    Western blot analysis of extracts of THP-1 cells, using PSMB10 antibody (A5452) at 1:5000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunohistochemistry - PSMB10 Polyclonal Antibody (A5452) }

    Immunohistochemistry - PSMB10 Polyclonal Antibody (A5452)

    Immunohistochemistry of paraffin-embedded rat brain using PSMB10 antibody (A5452) at dilution of 1:200 (40x lens).
    ABclonal:Immunohistochemistry - PSMB10 Polyclonal Antibody (A5452) }

    Immunohistochemistry - PSMB10 Polyclonal Antibody (A5452)

    Immunohistochemistry of paraffin-embedded mouse brain using PSMB10 antibody (A5452) at dilution of 1:200 (40x lens).
    ABclonal:Immunofluorescence - PSMB10 Polyclonal Antibody (A5452) }

    Immunofluorescence - PSMB10 Polyclonal Antibody (A5452)

    Immunofluorescence analysis of HeLa cells using PSMB10 antibody (A5452). Blue: DAPI for nuclear staining.

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