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RNASEL Rabbit pAb (A9840)

Publication (1) Datasheet

ABclonal:Western blot - RNASEL Rabbit pAb (A9840)

Overview

Product nameRNASEL Rabbit pAb
Catalog No.A9840
RRIDAB_2772029
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 472-741 of human RNASEL (NP_066956.1).
SequenceVQELHLSCGYTHQDLQPQNILIDSKKAAHLADFDKSIKWAGDPQEVKRDLEDLGRLVLYVVKKGSISFEDLKAQSNEEVVQLSPDEETKDLIHRLFHPGEHVRDCLSDLLGHPFFWTWESRYRTLRNVGNESDIKTRKSESEILRLLQPGPSEHSKSFDKWTTKINECVMKKMNKFYEKRGNFYQNTVGDLLKFIRNLGEHIDEEKHKKMKLKIGDPSLYFQKTFPDLVIYVYTKLQNTEYRKHFPQTHSPNKPQCDGAGGASGLASPGC
Gene ID6041
Swiss protQ05823
SynonymsRNASEL; PRCA1; RNS4
Calculated MW73kDa/83kDa
Observed MW84kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIF/ICCIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIP
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samples293T, HT-29, Jurkat
Cellular locationCytoplasm, Mitochondrion
Customer validation

WB(Homo sapiens)

    ABclonal:Western blot - RNASEL Rabbit pAb (A9840) }

    Western blot - RNASEL Rabbit pAb (A9840)

    Western blot analysis of extracts of various cell lines, using RNASEL antibody (A9840) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

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