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TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

Reviews (11)Publications (41) Datasheet

ABclonal:Western blot - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal: - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)

Overview

Product nameTriMethyl-Histone H3-K27 Rabbit pAb
Catalog No.A2363
RRIDAB_2756439
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic trimethylated peptide around K27 of human histone H3 (NP_003520.1).
SequenceAARKS
Gene ID8290
Swiss protQ16695
SynonymsH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW15kDa
Observed MW17KDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
ChIP-seq 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesMCF7 acid extract, 293T acid extract, C2C12 acid extract, NIH/3T3 acid extract, C6 acid extract, H3 protein
Cellular locationChromosome, Nucleus
Customer validation

(Human embryonic stem cells, A549, Mouse embryonic stem cells, Mouse lung tumor, Mouse E16.5 neocortex, Goat sperm, HEK293T, HeLa, Rice)

ChIP(Oryza sativa, Gossypium, Rattus norvegicus)

IHC(Sus scrofa, Mus musculus, Homo sapiens)

WB(Homo sapiens, Rattus norvegicus, Mus musculus)

ChIP(Homo sapiens, Gossypium)

IP(Gossypium)

IF(Homo sapiens, Gallus gallus, Mus musculus, Sus scrofa)

ChIP-qPCR(Mus musculus, Homo sapiens)

ChIP-Seq(Zea mays)

IP(Mus musculus)

CHIP(castor bean , Homo sapiens)

ChiP(Mus musculus)

IF(Homo sapiens)

    ABclonal:Western blot - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)}

    Western blot - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

    Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (A2363) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10032 dilution.
    Lysates/proteins: 57ug per lane.
    Blocking buffer: 35% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00052).
    Exposure time: 0.5s.
    ABclonal: - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)}

    - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

    Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (A2363).
    ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)}

    Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

    Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)}

    Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

    Immunohistochemistry of paraffin-embedded human breast cancer using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)}

    Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

    Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)}

    Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

    Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 Rabbit pAb (A2363) at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)}

    Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

    Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 Rabbit pAb (A2363) at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)}

    Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

    Immunofluorescence analysis of PC-12 cells using TriMethyl-Histone H3-K27 Rabbit pAb (A2363) at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)}

    Chromatin Immunoprecipitation - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

    Chromatin immunoprecipitation analysis of extracts of HeLa; cells, using TriMethyl-Histone H3-K27 Rabbit pAb antibody (A2363) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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