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ABScript III One Step RT-qPCR Probe Kit with UDG V5 (RK20412)

Datasheet

ABclonal:ABScript III One Step RT-qPCR Probe Kit with UDG V5
Summary

Catalog No.: RK20412

Background

ABScript III One Step RT-qPCR Probe Kit with UDG V5 is a ready-to-use kit allowing reverse transcription and subsequent probe-based qPCR in a single tube. It contains all components for RT-qPCR except primers, probes and RNA templates. The one-step format significantly improves sensitivity and effectively prevent contamination. The heat-liable UDG in this product could degrade U-contained contamination in room temperature, and inactivated in 50℃, which could prevent false positive results without affect the efficiency and sensitivity. The ABScript III Reverse Transcriptase in the kit provides reliable reverse transcription to a wide range of RNA template amount. After reverse transcription, the Hot-start version of Taq polymerase is activated at 95°C and the ABScript III Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5'-3' exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. The ABScript III One Step RT-qPCR Probe Kit is an ideal product for high-speed analyses of low input RNA sample

Product Components

2X One Step RT-qPCR Probe Buffer IVRM21475
One Step Probe Enzyme Mix IVRM21476
50X ROX Dye IRM21465
50X ROX Dye IIRM21466
Nuclease-free H2ORM20214

Product Information

ApplicationsOne Step probe RT-qPCR,RNA viruses detection
Storage TemperatureUpon receipt, store all components at -20°C.

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