Custom Mouse Monoclonal Antibody Production

Monoclonal antibodies (mAbs) have a much higher specificity and lower between-batch variability compared to pAbs.

Our custom mouse monoclonal antibody service adopts the hybridoma (fusion) technology to produce large numbers of highly specific monoclonal antibodies.

Antibody-secreting B cells are immortalized by merging B lymphocytes and myeloma cells in the immunized mouse. The hybridoma cell line secreting the monoclonal antibody is then selected using limited dilution cloning. The monoclonal antibody is produced on a large scale by amplifying the culture of the cell strain.


  • Double Verification – Our procedure focuses on testing the mouse bleeds by WB (endogenous lysates) besides ELISA for mouse selection and sub cloning screening step.
  • Guaranteed Results – For WB antibody

  • Antigen Option – Antigens can be provided by you or prepared by us.

Mouse Monoclonal Antibody Production Process


1-3 mg of purified antibody from best hybridoma line

Up to three of the best hybridoma cell lines

QA/QC report for the antigen if provided by ABclonal

100ug antigen if provided by ABclonal

Project Report