Monoclonal antibodies (mAbs) have a much higher specificity and lower between-batch variability compared to pAbs.
Our custom mouse monoclonal antibody service adopts the hybridoma (fusion) technology to produce large numbers of highly specific monoclonal antibodies.
Antibody-secreting B cells are immortalized by merging B lymphocytes and myeloma cells in the immunized mouse. The hybridoma cell line secreting the monoclonal antibody is then selected using limited dilution cloning. The monoclonal antibody is produced on a large scale by amplifying the culture of the cell strain.
Guaranteed Results – For WB antibodylearn more
1、 5 BALB/c mice will be immunized, and the best mouse will be chosen for fusion.
2、 1-3 positive clones will be delivered.
3、 1 clone will be used to make ascites followed by purification.
For application-specific antibody (IHC, IF, IP, ChIP)
1、 10 mice will be immunized, and the best 2 mice will be chosen for fusion.
2、 1-3 positive clones will be delivered.
3、 3-5 clones will be used to make ascites followed by purification.
1-3 mg of purified antibody from best hybridoma line
Up to three of the best hybridoma cell lines
QA/QC report for the antigen if provided by ABclonal
100ug antigen if provided by ABclonal
Project Report
