A rapid high-throughput parallel peptide synthesis platform that enables to synthesize a larger quantity of high purity peptides.
The peptide library is a large collection of related and diverse sequences used to identify critical motifs for protein function or protein recognition. These powerful tools have wide applications in numerous areas such as proteomics research, drug test, epitope identification, enzyme substrate identification, receptor-ligand screening, etc.
Truncation Library – Truncation peptide libraries are used to identify the shortest amino acid sequence needed for activity.
The library is constructed by systemically removing the flanking residues of the original peptide. If the essential amino acids are known (e.g., by alanine scanning), the direction of truncation can be tailored to maintain these residues and truncate from the opposite end.
Special Instructions
Enter the number of residues you wish to remove per truncation step from the N- and C-termini. A value of 1 will remove a single amino acid at a time. A value of 0 at either terminus will fix that end of the sequence.
Positional Library – Positional scanning is an important tool for peptide sequence optimization.
This library identifies an amino acid of interest at a single position and substitutes it with all other natural amino acids one at a time. Changes in activity identify the preferred amino acid residues at this position.
Special Instructions
Enter your target sequence as well as the position(s) of the amino acid to be substituted, counting from the N-terminus. A total of 20 sequences will be generated for each positional replacement.
Alanine Scanning Library – Alanine Scanning is an approach to identify specific amino acid residues responsible for a peptide’s activity.
Alanine is used to substitute each residue sequentially. Substitution of an essential amino acid results in a reduction in peptide activity, with the degree of reduction taken as a relative measure of the importance of the amino acid being substituted.
Special Instructions
Alanine is the smallest chiral amino acid and is therefore most commonly used as the scanning substitute. However, any other amino acid may be used. Please contact us directly for more information on alternate scanning strategies.
Scrambled Library – Scrambled libraries are constructed through permutation of the original peptide sequence.
Scrambled libraries are typically used as: 1) negative controls to show that a specific sequence rather than the amino acid composition is critical for activity; or 2) tools for finding new leads by creating a random screening library.
Special Instructions
This tool can generate a maximum of 1000 permutations of your original sequence. For larger sets or for complete libraries of all 3-mer or 4-mer peptides containing every possible amino acid combination and permutation, please contact us directly.
Overlapping Library – Overlapping peptide libraries are ideal for T-cell epitope identification because T cell epitopes are by their nature short linear peptides from the primary protein sequence.
Overlapping peptide libraries are also appropriate for scanning the primary sequence of proteins for linear, or "continuous", B-cell (antibody-defined) epitopes.
Special Instructions
The library generation process is defined by two parameters: peptide length and offset number which reflects the degree of overlap. The next to last peptide in each set (the 'orphan') is often shorter than the other peptides as it lies at the C-Terminus. The last peptide included in the set represents the orphan sequence plus one or more N-Terminal residues to bring it to the correct length.
Truncation Library – Truncation peptide libraries are used to identify the shortest amino acid sequence needed for activity.
The library is constructed by systemically removing the flanking residues of the original peptide. If the essential amino acids are known (e.g., by alanine scanning), the direction of truncation can be tailored to maintain these residues and truncate from the opposite end.
Special Instructions
Enter the number of residues you wish to remove per truncation step from the N- and C-termini. A value of 1 will remove a single amino acid at a time. A value of 0 at either terminus will fix that end of the sequence.
Positional Library – Positional scanning is an important tool for peptide sequence optimization.
This library identifies an amino acid of interest at a single position and substitutes it with all other natural amino acids one at a time. Changes in activity identify the preferred amino acid residues at this position.
Special Instructions
Enter your target sequence as well as the position(s) of the amino acid to be substituted, counting from the N-terminus. A total of 20 sequences will be generated for each positional replacement.
Alanine Scanning Library – Alanine Scanning is an approach to identify specific amino acid residues responsible for a peptide’s activity.
Alanine is used to substitute each residue sequentially. Substitution of an essential amino acid results in a reduction in peptide activity, with the degree of reduction taken as a relative measure of the importance of the amino acid being substituted.
Special Instructions
Alanine is the smallest chiral amino acid and is therefore most commonly used as the scanning substitute. However, any other amino acid may be used. Please contact us directly for more information on alternate scanning strategies.
Scrambled Library – Scrambled libraries are constructed through permutation of the original peptide sequence.
Scrambled libraries are typically used as: 1) negative controls to show that a specific sequence rather than the amino acid composition is critical for activity; or 2) tools for finding new leads by creating a random screening library.
Special Instructions
This tool can generate a maximum of 1000 permutations of your original sequence. For larger sets or for complete libraries of all 3-mer or 4-mer peptides containing every possible amino acid combination and permutation, please contact us directly.
Overlapping Library – Overlapping peptide libraries are ideal for T-cell epitope identification because T cell epitopes are by their nature short linear peptides from the primary protein sequence.
Overlapping peptide libraries are also appropriate for scanning the primary sequence of proteins for linear, or "continuous", B-cell (antibody-defined) epitopes.
Special Instructions
The library generation process is defined by two parameters: peptide length and offset number which reflects the degree of overlap. The next to last peptide in each set (the 'orphan') is often shorter than the other peptides as it lies at the C-Terminus. The last peptide included in the set represents the orphan sequence plus one or more N-Terminal residues to bring it to the correct length.
